Nonhuman Primate Core

Computational Biology Core

Small Animal Core

BSL4 Core

Pathogenesis Expression Core

Law, Policy and Ethics

Trans-Center Cores

National Small Molecule Screening Laboratory

RCE Communications

Nonhuman Primate Aerobiology Core

Passive immunotherapeutics for                             Select Agents

Pathogenesis Expression Core

Collaborating Institution: Arizona State University, Tempe, AZ

Director: Stephen A. Johnston, Ph.D.

Associate Director: Kathyrn Sykes, Ph.D. – ASU, Tempe, AZ

Description: The purpose of the Pathogenesis Expression Core in the WRCE is primarily to support biothreat research in the WRCE, and secondarily to support other RCEs and biothreat researchers. We do so by providing the services and technologies necessary to study pathogenesis and host genomics and proteomics. These services are provided largely at material cost recovery rates. The following support is provided:

Affymetix arrays. The relevant arrays available (human, rat, mouse) will be processed and analyzed.
Spotted oligo arrays. Human and mouse slides and oligo sets will be produced. Pathogen oligo arrays will be made upon request. Arrays for Bacillus anthracis and Yersinia pestis are also currently under production in the Center for Innovations in Medicine (CIM) at ASU.
RNA preparation and labeling. Robotic stations for RNA preparation, evaluation, labeling and hybridization will be maintained to produce high-quality data.
Computation. In collaboration with the Computational Biology Core, we will provide the latest in software for analysis of microarray data. We will also strive to incorporate the latest developments such as those from the CIM and Sandia National Laboratories.
Q-PCR. The Q-PCR sub-core will provide a centralized resource for the rapid and high-throughput (HTP) quantitation of transcripts using fluorescent, real-time, and reverse transcriptase-coupled quantitative PCR.
HTP antibody production. The antibody production sub-core will enable users easy access to large numbers of custom-made antibodies for use as specific binding reagents.
HTP Luminex system. We will offer researchers the Luminex platform to detect and quantitate a variety of cellular and serum protein markers.